effect of melatonin on the expression of apoptotic genes in vitrified-thawed spermatogonia stem cells type a of 6-day-old mice

Authors

mohammadreza gholami department of anatomy, lorestan university of medical sciences, khoramabad, iran

ghasem saki physiology research center, ahvaz jundishapur university of medical sciences (ajums), ahvaz, iran

masoud hemadi fertility, infertility and perinatology research center, ahvaz jundishapur university of medical sciences (ajums), ahvaz, iran

ali khodadadi department of immunology, ahvaz jundishapur university of medical sciences (ajums), ahvaz, iran

abstract

objective(s):  being secreted by the pineal gland, melatonin induces cell proliferation in normal cells and induced apoptosis in cancer cells. the purpose of this study was to investigate effects of melatonin on main components and the expression of apoptotic genes in vitrified-thawed testicular germ cells of 6- day–old mice.  materials and methods: testes of neonate balb/c mice were vitrified- thawed under standard condition with or without the addition of 100 μm melatonin to both vitrification and thawing solutions. subsequently, vitrified-thawed wholetestes were digested under standard condition and spermatogonial stem cells type a were separate in the suspension with cd90.1 (thy1.1+) micro beads. extraction of rna and synthesis of cdna was performed. expression levels of apoptotic genes (fas, p53, bcl-2 and bax) were determined using real-time pcr. results: with all genes being expressed, level of expression for fas was higher and for that of p-53 was lower than the remaining genes. conclusion: melatonin may cause apoptosis in cells being damaged under the influence of freezing thawing process. in order to examine the exact effects of melatonin on spermatogonia stem cells apoptosis, additional studies are required.

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Effect of Melatonin on the Expression of Apoptotic Genes in Vitrified-thawed Spermatogonia Stem Cells Type A of 6-Day-Old Mice

 Objective(s):  Being secreted by the pineal gland, melatonin induces cell proliferation in normal cells and induced apoptosis in cancer cells. The purpose of this study was to investigate effects of melatonin on main components and the expression of apoptotic genes in vitrified-thawed testicular germ cells of 6- day–old mice.  Materials and Methods: Testes of neonate Balb/c mice were vitrifie...

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Effect of Melatonin on the Expression of Apoptotic Genes in Vitrified-thawed Spermatogonia Stem Cells Type A of 6-Day-Old Mice

OBJECTIVE(S) Being secreted by the pineal gland, melatonin induces cell proliferation in normal cells and induced apoptosis in cancer cells. The purpose of this study was to investigate effects of melatonin on main components and the expression of apoptotic genes in vitrified-thawed testicular germ cells of 6- day-old mice. MATERIALS AND METHODS Testes of neonate Balb/c mice were vitrified- t...

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Journal title:
iranian journal of basic medical sciences

جلد ۱۶، شماره ۸، صفحات ۹۰۶-۹۰۹

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